![]() How long will my sample last after mounting?: The FAM signal should be imaged immediately. Please note that FAM is incompatible with enzymatic glucose-oxidase/catalase (GLOX) antifade. Usage should be limited to specimens with known low autofluorescence, fixed with MeOH/HAC, and when other filter options are not available.Īnti-fade Recommendations: The FAM dye requires an anti-fade mounting media and is compatible with Vectashield by Vector Labs (H-1000) and Prolong Diamond by ThermoFisher (P36961). Additionally we recommend the use of FAM-labeled probe sets with >35 individual probes, and as a compliment to a primary probe set. Because of this, probe labeled with FAM perform best in cultured cells. This auto-fluorescence can make it difficult to discern true RNA transcripts from background, and is especially true in tissue where auto-fluorescence is generally already higher. Special Notes and Considerations: The green channel where FAM fluoresces is not ideal for RNA FISH as there tends to be high auto-fluorescence from the specimen itself. ![]() FAM fluoresces in the green region of the visible spectrum. ![]() ![]() General Description: The Fluorescein modification incorporates a fluorescein (FAM) moiety at the terminus of an oligonucleotide. ![]()
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